Prof.
Christophe
TATOUT
Professor of Plant epigenetics and bioinformatics
ORCID link : 0000-0001-5215-2338
Website links : Research Group
IRP : OMERO embedding and Optimisation of Biom3d, an image analysis tool using deep-learning networks

iGRED, Clermont-Ferrand, France
Chromatin and nuclear dynamics
My research focuses on developing standardized, FAIR-compliant workflows for bioimage data handling from acquisition and storage to analysis. Using OMERO as a data management platform, integrated with HPC resources at our data center, I develop methods of image analysis on plant nuclei microscopy datasets to study chromatin and nuclear dynamics in plants. Our recent developments focused on a reproducible 3D semantic segmentation and robust nuclear morphology analysis within an all-in-one bioimaging environment called biom3d, a new framework based on deep-learning architecture.
Five Main Publications
Biom3d, a modular framework to host and develop 3D segmentation methods
Mougeot G, Safarbati S, Alégot H, Pouchin P, Field N, Almagro S, Pery E, Probst AV, Tatout C, Evans DE, Graumann K, Chausse F, Desset S. (2024) bioRxiv 2024.07.25.604800, https://doi.org/10.1101/2024.07.25.604800i in révision in Medical Image Analysis
Image analysis workflows to reveal the spatial organization of cell nuclei and chromosomes
Randall RS, Jourdain C, Nowicka A, Kaduchová K, Kubová M, Ayoub MA, Schubert V, Tatout C , Colas I, Kalyanikrishna, Desset S , Mermet S , Boulaflous-Stevens A, Kubalová I, Mandáková T, Heckmann S, Lysak MA, Panatta M, Santoro R, Schubert D, Pecinka A, Routh D, Baroux C (2022). Nucleus, 13: 277-299
Journal of Cell Science
Mougeot G., Dubos T., Chausse F., Péry E., Graumann K., Tatout C., Evans DE. and Desset S. (2022) Journal of Cell Science, 135, jcs258986
NODeJ: an ImageJ plugin for 3D segmentation of nuclear objects
Dubos, A Poulet, G Thomson, E Péry, F Chausse, C Tatout, S Desset, van Wolfswinkel J.C., Yannick Jacob Y. (2022) BMC bioinformatics, 23: 216
Automated 3D bio-imaging analysis of nuclear organisation by NucleusJ 2.0
Dubos T., Poulet A., Gonthier-Gueret C., Mougeot G., Vanrobays E., Li L., Tutois S., Pery E. Chausse F., Probst A.V., Tatout C. and Desset S (2020). Nucleus, 11: 315-329

