Martina
DVOŘÁČKOVÁ
Senior researcher
Chromatin Molecular Complexes, group of J. Fajkus
Mendel center for plant genomics and proteomics
ORCID link : 0000-0001-5998-6159
Website links : Profil
CEITEC MU, Brno, Czech Republic
chromatin stability, DNA repair during cell cycle
We focus on how plant cells maintain genome integrity, through the coordinated regulation of DNA repair, chromatin assembly, and cell cycle progression. Using advanced live-cell imaging, we visualize DNA repair processes in real time across defined cell cycle phases, employing Arabidopsis PCNA1 as a marker of the early DNA damage response in wild-type plants and mutants defective in key DNA repair and chromatin-associated proteins. We monitor the recruitment of repair factors to sites of DNA damage by laser microirradiation and combine it with high-resolution microscopy approaches. In parallel, we examine nucleolar responses to DNA lesions to link genome maintenance with cellular metabolism and nuclear organization, and assess the stability of repetitive genomic elements, which often reflect altered DNA repair processing under mutant or genotoxic stress conditions
Five Main Publications
G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability
Eekhout T, Dvorackova M, Pedroza Garcia JA, Nespor Dadejova M, Kalhorzadeh P, Van den Daele H, Vercauteren I, Fajkus J, De Veylder L (2021) Plant Physiol 186: 1893-1907
In-section Click-iT detection and super-resolution CLEM analysis of nucleolar ultrastructure and replication in plants
Franek M, Koptasikova L, Miksatko J, Liebl D, Macickova E, Pospisil J, Esner M, Dvorackova M, Fajkus J (2024) Nat Commun 15: 2445
Histone Chaperone Deficiency in Arabidopsis Plants Triggers Adaptive Epigenetic Changes in Histone Variants and Modifications
Franek M, Nespor Dadejova M, Pirek P, Krystofova K, Dobisova T, Zdrahal Z, Dvorackova M, Lochmanova G (2024) Mol Cell Proteomics 23: 100795
Disruption of NAP1 genes in Arabidopsis thaliana suppresses the fas1 mutant phenotype, enhances genome stability and changes chromatin compaction
Kolarova K, Nespor Dadejova M, Loja T, Lochmanova G, Sykorova E, Dvorackova M (2021) Plant J 106: 56-73
Laser microirradiation as a versatile system for probing protein recruitment and protein-protein interactions at DNA lesions in plants
Nespor Dadejova M, Franek M, Dvorackova M (2022) New Phytol 234: 1891-1900